Alteration of substrate specificity of alanine dehydrogenase
نویسندگان
چکیده
منابع مشابه
Substrate specificity of the pyruvate dehydrogenase complex from Escherichia coli.
The investigation of the substrate specificity of the pyruvate dehydrogenase complex from Escherichia coli allows a description of the binding region of pyruvate. Substrate analogs with electronegative substitutions in the methyl group show a strong competitive inhibition of the overall reaction of the pyruvate dehydrogenase complex. The most efficient inhibitor is fluoropyruvate which has a mo...
متن کامل17 -Hydroxysteroid dehydrogenase of rat skin. Substrate specificity and inhibitors.
A 17fl-hydroxysteroid dehydrogenase activity has been demonstrated in the microsomal preparations made from the skin of newborn rats. The enzyme prefers NAD to NADP as the coenzyme and utilizes 17fi-estradiol as well as testosterone as substrates. The apparent K, values estimated for 17/?-estradiol are 4.1 X 10m6 M, for estrone 1.0 X 10d5 M, and for testosterone 4.0 X 10e6 M. The enzyme can be ...
متن کاملAlteration of the specificity of malate dehydrogenase by chemical modulation of an active site arginine.
Malate dehydrogenase from Escherichia coli is highly specific for the oxidation of malate to oxaloacetate. The technique of site-specific modulation has been used to alter the substrate binding site of this enzyme. Introduction of a cysteine in place of the active site binding residue arginine 153 results in a mutant enzyme with diminished catalytic activity, but with K(m) values for malate and...
متن کاملAlteration of coenzyme specificity of malate dehydrogenase from Streptomyces coelicolor A3(2) by site-directed mutagenesis.
We describe here for the first time the alteration of coenzyme specificity of malate dehydrogenase (MDH) from Streptomyces coelicolor A3(2) (ScMDH). In the present study, we replaced four amino acid residues in the Rossmann fold (βB-αC) region of NADH-dependent ScMDH by site-directed mutagenesis with those of NADPH-dependent MDH (Glu42Gly, Ile43Ser, Pro45Arg, and Ala46Ser). The coenzyme specifi...
متن کاملThe Role of Herpes Simplex Virus-1 Thymidine Kinase Alanine 168 in Substrate Specificity
Herpes simplex virus type 1 (HSV) thymidine kinase (TK) has been widely used in suicide gene therapy for the treatment of cancer due to its broad substrate specificity and the inability of the endogenous human TK to phosphorylate guanosine analogs such as ganciclovir (GCV). The basis of suicide gene therapy is the introduction of a gene that encodes a prodrug-activating enzyme into tumor cells....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 2014
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/gzu053